Journal: EBioMedicine

Article Title: Radiotherapy Upregulates Programmed Death Ligand-1 through the Pathways Downstream of Epidermal Growth Factor Receptor in Glioma

doi: 10.1016/j.ebiom.2018.01.027

Figure Lengend Snippet: The effect of PD-L1 blockade on the suppressing ability of tumor cells for CD8 + T cell proliferation. Cell lines (U87 and U251) were treated with RT (5 Gy), PD-L1 Ab (20 μg/mL), RT + PD-L1 Ab, AG490 (10 μM), RT + AG490, or RT + AG490 + PD-L1 Ab, and then co-cultured with CD8 + T cells. At 48 h, CD8 + T cell proliferation was evaluated by FCM. Non-specific Stimulation: anti-CD3/CD28 beads. Representative images and quantitative data are shown. Each column is shown as the means of three separate experiments; bars, SD. Student's t -test; *** P < 0.001. ANOVA; ns, nonsignificant.

Article Snippet: CD8 + T cell immunomagnetic beads positive selection kit was purchased from Stem-Cell Tech (USA).

Techniques: Cell Culture

Journal: EBioMedicine

Article Title: Radiotherapy Upregulates Programmed Death Ligand-1 through the Pathways Downstream of Epidermal Growth Factor Receptor in Glioma

doi: 10.1016/j.ebiom.2018.01.027

Figure Lengend Snippet: The effect of PD-L1 blockade on the cytotoxicity of CD8 + T cells against glioma cells. Cell lines (U87 and U251) were treated with RT (5 Gy), PD-L1 Ab (20 μg/mL), RT + PD-L1 Ab, AG490 (10 μM), RT + AG490, or RT + AG490 + PD-L1 Ab, and then co-cultured with CD8 + T cells. At 48 h, tumor cell apoptosis was evaluated by FCM. Representative images and quantitative data are shown. Each column is shown as the means of three separate experiments; bars, SD. Student's t-test; *** P < 0.001. ANOVA; ns, nonsignificant.

Article Snippet: CD8 + T cell immunomagnetic beads positive selection kit was purchased from Stem-Cell Tech (USA).

Techniques: Cell Culture

Journal: Scientific Reports

Article Title: Impaired Mobilization of Vascular Reparative Bone Marrow Cells in Streptozotocin-Induced Diabetes but not in Leptin Receptor-Deficient db/db Mice

doi: 10.1038/srep26131

Figure Lengend Snippet: Representative dot plots of flow cytometric analysis of LK cells that were enriched by immunomagnetic selection as described in the text: ( A ) Sample treated with isotype controls, and ( C ) sample treated with fluorescent-conjugated antibodies. ( C ) Gating strategy for the selection of LK cells. Sequential gating involves exclusion of dead cells, stained by 7-AAD, exclusion of doublets or cell clumps, selection of Lineage negative (Lin − ) cells (exclusion of differentiated cells) and then selection of c-Kit + cells (LK). Note the presence a small population of LK cells that are positive Sca-1, and Lin − cells that are not expressing Sca-1 or c-Kit.

Article Snippet: Lineage-negative (Lin − ) population of cells was isolated by negative selection by using immunomagnetic enrichment kit (StemCell Technologies) as per the manufacturer’s instructions.

Techniques: Selection, Staining, Expressing